MAP Publications

Accelerated Wound Healing by Injectable Microporous Gel Scaffolds Assembled from Annealed Building Blocks

Nature Materials

Injectable hydrogels can provide a scaffold for in situ tissue regrowth and regeneration, however these injected materials require gel degradation prior to tissue reformation limiting their ability to provide physical support. We have created a new class of injectable biomaterial that circumvents this challenge by providing an interconnected microporous network for simultaneous tissue reformation and material degradation. We assemble monodisperse micro-gel building blocks into an interconnected microporous annealed particle (MAP) scaffold . . .

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Particle Hydrogels Based on Hyaluronic Acid Building Blocks

ACS Biomaterials Science & Engineering

...we explore three orthogonal annealing chemistries based on an enzymatic reaction, light based radical polymerization, and amine/carboxylic acid based cross-linking to demonstrate the versatility of our particle hydrogels and explore potential physical differences between the approaches. We explore the connectivity of the generated pores, the pore area/void fraction of the resulting scaffold, the mechanical properties of the scaffold, and cell spreading within scaffolds formed with the three different annealing mechanisms . . .

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Injectable and Spatially Patterned Microporous Annealed Particle (MAP) Hydrogels for Tissue Repair Applications

Advanced Science

Here, an injectable spatially patterned hydrogel is reported using hyaluronic acid (HA)-based particle hydrogels. These particle hydrogels are sequentially loaded into a syringe to form a pattern and, once injected, they maintain the pattern. The applicability of this hydrogel in a wound healing skin model, a subcutaneous implant model, as well as a stroke brain model is examined and distinct patterning in all models tested is shown . . .

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Injection of Microporous Annealing Particle (MAP) Hydrogels in the Stroke Cavity Reduces Gliosis and Inflammation and Promotes NPC Migration to the Lesion

Advanced Materials

Here, this paper shows that the injection of a porous hyaluronic acid hydrogel into the stroke cavity significantly reduces the inflammatory response following stroke while increasing peri-infarct vascularization compared to nonporous hydrogel controls and stroke only controls. In addition, it is shown that the injection of this material impacts NPCs proliferation and migration at the subventricular zone niche and results, for the first time, in NPC migration into the stroke site . . .

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Granular Hydrogels: Emergent Properties of Jammed Hydrogel Microparticles and their Applications in Tissue Repair and Regeneration

Current Opinion in Biotechnology

Granular hydrogels are emerging as a versatile and effective platform for tissue engineered constructs in regenerative medicine. The hydrogel microparticles (HMPs) that compose these materials exhibit particle jamming above a minimum packing fraction, which results in a bulk, yet dynamic, granular hydrogel scaffold. These injectable, microporous scaffolds possess self-assembling, shear-thinning, and self-healing properties . . .

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Scalable High‐Throughput Production of Modular Microgels for In Situ Assembly of Microporous Tissue Scaffolds

Advanced Functional Materials

...manufacturing throughput, reproducibility, and flexibility of hydrogel microparticle building blocks are limited, hindering widespread adoption. The scalable high‐throughput production of bioactive microgels for the formation of microporous tissue scaffolds in situ is presented. Using a parallelized step emulsification device, scalable high‐throughput generation of monodisperse microgels is achieved . . .

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